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anti total ptk2 fak antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti total ptk2 fak antibody
    Anti Total Ptk2 Fak Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fak+antibody/FAK+Antibody/pm42887180-338-3-6
    Average 96 stars, based on 1713 article reviews
    anti total ptk2 fak antibody - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Staining:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Control:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Marker:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Migration:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Scratch Wound Assay Assay:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Western Blot:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Chromatin Immunoprecipitation:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Flow Cytometry:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Magnetic Resonance Imaging:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Biomarker Discovery:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.

    Ubiquitin Proteomics:

    Article Title: Salmonella Typhimurium manipulates macrophage cholesterol homeostasis through the SseJ-mediated suppression of the host cholesterol transport protein ABCA1.
    Article Snippet: Funding information Center for Scientific Review, Grant/Award Number: RO1DK58536 Abstract Upon infection of host cells, Salmonella enterica serovar Typhimurium resides in a modified-endosomal compartment referred to as the Salmonella-containing vacuole (SCV).. SCV biogenesis is driven by multiple effector proteins translocated through two type III secretion systems (T3SS-1 and T3SS-2).. While many host proteins targeted by these effector proteins have been characterised, the role of host lipids in SCV dynamics remains poorly understood.

    Article Title: Phillygenin Improves the Focal Adhesion Kinase/Glycogen Synthase Kinase 3β/β-Catenin Axis to Promote Mesenchymal Stem Cell Osteogenic Differentiation.
    Article Snippet: Cell Signaling Technology (Danvers, MA, USA) provided Runt- Related Transcription Factor 2 (RUNX2), Phospho- FAK (Tyr397) (pFAKY397), Glycogen Synthase Kinase 3β (GSK- 3β), PhosphoGlycogen Synthase Kinase 3β (pGSK- 3β), and β- catenin antibody.

    Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
    Article Snippet: The following reagents were obtained from Invitrogen: Alexa Fluor-568 and Alexa Fluor-488 donkey anti-rabbit IgG (H&L), Alexa Fluor-488 and Alexa Fluor-568 donkey anti-mouse IgG (H&L), and F-actin dye.

    Article Title: Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression.
    Article Snippet: Antibodies used for WB were as follows: COUP‐TFII_V1 (cat. no. ab41859; 1:500 dilu‐ tion; Abcam), COUP‐TFII (cat. no ab50487; 1:1,000 Abcam), β‐catenin (cat. no. sc‐7963; 1:1,000; Santa Cruz Biotechnology, Inc.); ERK (cat. no. sc‐94; 1:100; Santa Cruz Biotechnology, Inc.); phosphorylated (P)‐ERK (cat. no. sc‐7383; 1:200; Santa Cruz Biotechnology, Inc.); AKT (cat. no. 9272; 1:1,000; Cell Signaling Technology, Inc.); P‐AKT (cat. no. 9271; 1:1,000; Cell Signaling Technology, Inc.); AMPK (cat. no. 2603; 1:1,000; Cell Signaling Technology, Inc.); P‐AMPK (cat. no. 4188; 1:1,000; Cell Signaling Technology, Inc.); forkhead box (FOX)O3a (cat. no. ab47409; 1:1,000; Abcam); P‐FOXO3a (cat. no. ab47285; 1:1,000; Abcam); P‐GSK3 (cat. no. 8566; 1:1,000; Cell Signaling Technology, Inc.); GSK3 (cat. no. 5676; 1:1,000; Cell Signaling Technology, Inc.); vimentin (cat. no. M0725; 1:1,000; DAKO); GAPDH (cat. no. G8759; 1:10,000; MilliporeSigma); BRG1 (cat. no. ab70558; 1:1,000; Abcam); Histone H3 (cat. no. Ab10799, 1:1,000, Abcam); HSP70 (cat. no. sc‐24; 1:1,000; Santa Cruz Biotechnology, Inc.); FAK (cat. no. sc‐558; 1:200; Santa Cruz Biotechnology, Inc.); Vinculin (cat. no. V9131; 1:1,000; MilliporeSigma); P21 (cat. no. ab7960; 1:200; Abcam); pP21 (cat. no. ab47300; 1:500; Abcam); RhoA (cat. no. sc‐418; 1:100; Santa Cruz Biotechnology, Inc.); Ubiquitin (cat. no. SPA‐203; 1:1,000; Stressgene Corp.); β‐tubulin (cat. no. T5201, 1:500, Sigma).

    Article Title: In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regeneration
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used FAK (1:200; Santa Cruz Biotechnology; sc-558) pFAK (1:200; Santa Cruz Biotechnology; sc-16662) ERK (1:250; Abcam; ab9363) pERK (1:1000; Bio Legend; 919301) 3 nature portfolio | reporting sum m ary M arch 2021 p38 (1:1000; Abcam; ab31828) pp38 (1:1000; Abcam; ab4822) GAPDH (1:1000; Thermo Fisher Scientific; MA5-15738) Anti-mouse IgG-HRP (1:1000; Cell signaling; 7076) Goat anti-rabbit IgG-HRP (1:5000; Santa Cruz Biotechnology; sc-2004) Goat anti-mouse IgG-HRP(1:5000; Santa Cruz Biotechnology; sc-2005) Rabbit anti-goat lgG H&L-HRP(1:5000; Abcam; ab6741) Anti-Integrin beta 1 (1:100; Abcam; ab78502) Integrin α2 (1:100; Santa Cruz Biotechnology; sc-53353) Integrin α11 (1:100; Santa Cruz Biotechnology; sc-390091) Goat anti-mouse IgG H&L-DyLight 488 (1:500; Abcam; ab96879) goat anti-mouse IgG H&L (1:500; Abcam; ab97147) GFP (1:200; Santa Cruz Biotechnology; sc-9996) Anti-collagen type II (1:200; Abcam; ab34712), Collagen I (1:100; Novus Biologicals; NBP2-46874) Goat anti-Rabbit lgG (H+L) DyLight 594 (1:1000; Invitrogen; 35561) Validation Data provided in the manuscript.



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    Image Search Results


    Q209R preferentially engages FAK‐YAP signaling. (A) Western blot analysis of GNAQ, BAP1, Melan‐A, p‐ERK1/2 (Thr202/Tyr204), ERK1/2, FRA1, p‐AKT (Ser473), AKT, p‐4EBP1 (Ser65), and 4EBP1 expression in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (B) Quantification of the relative ratio between p‐AKT (Ser473) and AKT in Q209R and Q209L UM cell lines from (A). (C) Quantification of the relative ratio between p‐4EBP1 (Ser65) and 4EBP1 in Q209R and Q209L UM cell lines from (A). (D) Western blot analysis of p‐FAK (Tyr397), FAK, p‐YAP (Ser127), and YAP in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (E) Quantification of the relative ratio between p‐FAK (Tyr397) and FAK in Q209R and Q209L UM cell lines from (D). (F) Quantification of the relative ratio between p‐YAP (Ser127) and YAP in Q209R and Q209L UM cell lines from (D). Orthotopic tumor‐derived cell lines (QBM12.1 [Q209R], QBM12.3 [Q209R], QBM24 [Q209R], QBM45 [Q209L], QBM47 [Q209L]) and liver metastatic lines (QBM12L [Q209R], QBM12L2 [Q209R], QBM51L [Q209L], QBM53L [Q209L]) were used in A–F. (G) Representative IHC images showing YAP expression in primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (H) Quantification of the nuclear‐to‐cytoplasmic YAP intensity ratio (H‐score) among primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (I) Representative IHC images showing YAP expression in liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . (J) Quantification of nuclear YAP intensity (H‐score) among liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L .

    Journal: Pigment Cell & Melanoma Research

    Article Title: Allele‐Specific GNAQ Q209 Mutations Reveal Context‐Dependent Oncogenic Programs in Uveal Melanoma

    doi: 10.1111/pcmr.70121

    Figure Lengend Snippet: Q209R preferentially engages FAK‐YAP signaling. (A) Western blot analysis of GNAQ, BAP1, Melan‐A, p‐ERK1/2 (Thr202/Tyr204), ERK1/2, FRA1, p‐AKT (Ser473), AKT, p‐4EBP1 (Ser65), and 4EBP1 expression in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (B) Quantification of the relative ratio between p‐AKT (Ser473) and AKT in Q209R and Q209L UM cell lines from (A). (C) Quantification of the relative ratio between p‐4EBP1 (Ser65) and 4EBP1 in Q209R and Q209L UM cell lines from (A). (D) Western blot analysis of p‐FAK (Tyr397), FAK, p‐YAP (Ser127), and YAP in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (E) Quantification of the relative ratio between p‐FAK (Tyr397) and FAK in Q209R and Q209L UM cell lines from (D). (F) Quantification of the relative ratio between p‐YAP (Ser127) and YAP in Q209R and Q209L UM cell lines from (D). Orthotopic tumor‐derived cell lines (QBM12.1 [Q209R], QBM12.3 [Q209R], QBM24 [Q209R], QBM45 [Q209L], QBM47 [Q209L]) and liver metastatic lines (QBM12L [Q209R], QBM12L2 [Q209R], QBM51L [Q209L], QBM53L [Q209L]) were used in A–F. (G) Representative IHC images showing YAP expression in primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (H) Quantification of the nuclear‐to‐cytoplasmic YAP intensity ratio (H‐score) among primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (I) Representative IHC images showing YAP expression in liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . (J) Quantification of nuclear YAP intensity (H‐score) among liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L .

    Article Snippet: Antibodies against GNAQ (CST, #14373, RRID:AB_2665457), BAP1 (Abcam, ab255611), Melan‐A (Abcam, ab210546, RRID:AB_2889292), ERK1/2 (CST, #4695, RRID:AB_390779), p‐ERK1/2 (Thr202/Tyr204) (CST, #4370, RRID:AB_2315112), FRA1 (Abcam, ab252421, RRID:AB_2904513), AKT (CST, #4691, RRID:AB_915783), p‐AKT (Ser473) (CST, #4060, RRID:AB_2315049), p‐FAK (Tyr397) (CST, #8556, RRID:AB_10891442), FAK (CST, #3285, RRID:AB_2269034), 4E‐BP1 (CST, #9452, RRID:AB_331692), p‐4E‐BP1 (Ser65; CST, #9451, RRID:AB_330947), YAP (CST, #4912, RRID:AB_2218911), and p‐YAP (Ser127) (CST, #4911, RRID:AB_2218913) were used at 1:1000 as indicated.

    Techniques: Western Blot, Analysis, Expressing, Control, Derivative Assay, Transplantation Assay

    Q209R preferentially engages FAK‐YAP signaling. (A) Western blot analysis of GNAQ, BAP1, Melan‐A, p‐ERK1/2 (Thr202/Tyr204), ERK1/2, FRA1, p‐AKT (Ser473), AKT, p‐4EBP1 (Ser65), and 4EBP1 expression in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (B) Quantification of the relative ratio between p‐AKT (Ser473) and AKT in Q209R and Q209L UM cell lines from (A). (C) Quantification of the relative ratio between p‐4EBP1 (Ser65) and 4EBP1 in Q209R and Q209L UM cell lines from (A). (D) Western blot analysis of p‐FAK (Tyr397), FAK, p‐YAP (Ser127), and YAP in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (E) Quantification of the relative ratio between p‐FAK (Tyr397) and FAK in Q209R and Q209L UM cell lines from (D). (F) Quantification of the relative ratio between p‐YAP (Ser127) and YAP in Q209R and Q209L UM cell lines from (D). Orthotopic tumor‐derived cell lines (QBM12.1 [Q209R], QBM12.3 [Q209R], QBM24 [Q209R], QBM45 [Q209L], QBM47 [Q209L]) and liver metastatic lines (QBM12L [Q209R], QBM12L2 [Q209R], QBM51L [Q209L], QBM53L [Q209L]) were used in A–F. (G) Representative IHC images showing YAP expression in primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (H) Quantification of the nuclear‐to‐cytoplasmic YAP intensity ratio (H‐score) among primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (I) Representative IHC images showing YAP expression in liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . (J) Quantification of nuclear YAP intensity (H‐score) among liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L .

    Journal: Pigment Cell & Melanoma Research

    Article Title: Allele‐Specific GNAQ Q209 Mutations Reveal Context‐Dependent Oncogenic Programs in Uveal Melanoma

    doi: 10.1111/pcmr.70121

    Figure Lengend Snippet: Q209R preferentially engages FAK‐YAP signaling. (A) Western blot analysis of GNAQ, BAP1, Melan‐A, p‐ERK1/2 (Thr202/Tyr204), ERK1/2, FRA1, p‐AKT (Ser473), AKT, p‐4EBP1 (Ser65), and 4EBP1 expression in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (B) Quantification of the relative ratio between p‐AKT (Ser473) and AKT in Q209R and Q209L UM cell lines from (A). (C) Quantification of the relative ratio between p‐4EBP1 (Ser65) and 4EBP1 in Q209R and Q209L UM cell lines from (A). (D) Western blot analysis of p‐FAK (Tyr397), FAK, p‐YAP (Ser127), and YAP in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (E) Quantification of the relative ratio between p‐FAK (Tyr397) and FAK in Q209R and Q209L UM cell lines from (D). (F) Quantification of the relative ratio between p‐YAP (Ser127) and YAP in Q209R and Q209L UM cell lines from (D). Orthotopic tumor‐derived cell lines (QBM12.1 [Q209R], QBM12.3 [Q209R], QBM24 [Q209R], QBM45 [Q209L], QBM47 [Q209L]) and liver metastatic lines (QBM12L [Q209R], QBM12L2 [Q209R], QBM51L [Q209L], QBM53L [Q209L]) were used in A–F. (G) Representative IHC images showing YAP expression in primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (H) Quantification of the nuclear‐to‐cytoplasmic YAP intensity ratio (H‐score) among primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (I) Representative IHC images showing YAP expression in liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . (J) Quantification of nuclear YAP intensity (H‐score) among liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L .

    Article Snippet: Antibodies against GNAQ (CST, #14373, RRID:AB_2665457), BAP1 (Abcam, ab255611), Melan‐A (Abcam, ab210546, RRID:AB_2889292), ERK1/2 (CST, #4695, RRID:AB_390779), p‐ERK1/2 (Thr202/Tyr204) (CST, #4370, RRID:AB_2315112), FRA1 (Abcam, ab252421, RRID:AB_2904513), AKT (CST, #4691, RRID:AB_915783), p‐AKT (Ser473) (CST, #4060, RRID:AB_2315049), p‐FAK (Tyr397) (CST, #8556, RRID:AB_10891442), FAK (CST, #3285, RRID:AB_2269034), 4E‐BP1 (CST, #9452, RRID:AB_331692), p‐4E‐BP1 (Ser65; CST, #9451, RRID:AB_330947), YAP (CST, #4912, RRID:AB_2218911), and p‐YAP (Ser127) (CST, #4911, RRID:AB_2218913) were used at 1:1000 as indicated.

    Techniques: Western Blot, Analysis, Expressing, Control, Derivative Assay, Transplantation Assay